Details
Sample Preparation and Characterization Core Facility (SPC)
The SPC core facility at EMBL Hamburg offers a pipeline from the lab bench to the beam lines',
helping you to optimize and
prepare samples for structural studies. Our high-throughput crystallization
laboratory offers initial crystallization screens
as well as customized screens for optimization of initial
hits, both suitable for soluble and membrane proteins. Online observation
of the plates is possible via
the Crystallization Information Management System (CRIMS), which makes results available to
users
in real-time, along with all experimental parameters of the crystallization condition. Integration of
crystallization
and synchrotron data collection facilities can be implemented through automated crystal
harvesting and processing. In addition,
we offer assistance to perform SAXS batch measurements
with near-real time outputs of macromolecular structural parameters
and low-resolution solution-state
structures. The biophysical platform of the SPC includes cutting-edge technologies to measure
interactions
and to precisely determine the stability, shape and size of different biomolecules and
biomolecular assemblies identifying
the most suitable biophysical techniques to answer the biological
questions the researchers are trying to tackle.
Address: Notkestraße 85, Gebäude 25a
22607 Hamburg
Hamburg
Deutschland
To website
22607 Hamburg
Hamburg
Deutschland
To website
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Host Institution
European Molecular Biology Laboratory (EMBL)
Notkestraße 85, Gebäude 25a
22607 Hamburg
Hamburg
Deutschland
http://www.embl-hamburg.de/services/spc/index.html
Notkestraße 85, Gebäude 25a
22607 Hamburg
Hamburg
Deutschland
http://www.embl-hamburg.de/services/spc/index.html
Centre for Structural Systems Biology (CSSB)
Notkestraße 85, Gebäude 15
22607 Hamburg
Hamburg
Deutschland
https://www.cssb-hamburg.de/facilities/index_eng.html
Notkestraße 85, Gebäude 15
22607 Hamburg
Hamburg
Deutschland
https://www.cssb-hamburg.de/facilities/index_eng.html
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Scientific Domain
Primary Subjects:
- Biology
Secondary Subjects:
- Chemistry
- Physics
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Category
Systems Biology/Computational Biology Facilities
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Scientific Services
EMBL Hamburg has established a fully automated platform for the high-throughput crystallization of
biological macromolecules
in order to address a common bottleneck in x-ray crystallography. The SPC
crystallisation platform offers a wide range of
crystallisation screening conditions and flexible methods
have been developed to adapt each step to individual project requirements.
The services are available
to the general user community. Our crystallisation platform supports both nanodrop crystallisation
and
lipidic cubic phase (LCP) crystallisation. All plates can be imaged in one of our Rockimaker 1000
imagers (4º or 19 ºC)
and images accessed off-site through CRIMS. The facility is well supported with
two Mosquito-LCP at room temperature. Custom
screens can be formulated using automated
dispensing robots. Moreover, our Molecular Biophysics platform offers one of the
most-well equipped
biophysical laboratories in Europe. We support users with the design, execution and data analysis of
biophysical
experiments aimed at the characterization of proteins, protein complexes and interactions
between proteins and other types
of molecules. The characterization of these structures are analysed
by studying parameters such as stability, shape, folding
state, aggregation tendency, homogeneity
and oligomeric states. The interactions in which they are involved can be followed
by measuring
biophysical criteria like; specificity, kinetics, affinity, and thermodynamics.
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Scientific Equipment
- Mosquito LCP crystallization robots and online follow
- CrystalDirect Automatic crystal harvester
- Scorpion Screen Formulator and Biomek automated
- Microcal VP-ITC and PEAQ-ITC
- Microscale Thermophoresis Labelled and Label Free
- nano Differential Scattering Fluorescence (nDSF)
- Circular Dichroism spectrophotometer
- Bruker Vertex 70 Fourier-transform infrared spectrophometer
- MALDI-TOF with High Mass detector
- Biolayer Interferometry Octet RED96
- SX-20 Stopped-Flow spectrophometer
- Dynamic Light Scattering (DLS)
- SPR T200 (Biacore)
- SAXS-batch mode
- Molecular Dynamics simulations
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Keywords
- Structural Biology
- Molecular Biophysics
- Crystallization
- Stability
- Homogeneity
- Thermodynamics
- Kinetics
- Structure and Function
- Protein interactions
- Scattering
- Binding
- Complexes
- Aggregation
- Protein Folding and Unfolding
- Data Analysis
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Networks
iNEXT- Discovery
https://inext-discovery.eu/network/inext-d/home
https://inext-discovery.eu/network/inext-d/home
Instruct-ERIC
https://instruct-eric.eu/
https://instruct-eric.eu/
Association of Resources for Biophysical Research in Europe (ARBRE-MOBIEU)
https://arbre-mobieu.eu/
https://arbre-mobieu.eu/
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Users per annum
Internal Users: 57 (2019)
External Users in total: 177 (2019)
External Users: 112 (2019)
External Users in the EU: 67 (2019)
External Users outside of EU: 55 (2019)