Advances in stem cell research provide novel in vitro models of human disease and avenues for cell therapeutic approaches. The Freiburg iPS Core (FiPS) represents the centralized facility for the generation and provision of human induced pluripotent stem (iPS) cells at the University of Freiburg. In addition to derivation, maintenance, expansion and cryopreservation of human iPS cell lines, the iPS core facility offers support to researchers and physicians in the pursuit of projects involving iPS cells, including differentiation paradigms and 3D organoid culture. Differentiation protocols are established for a range of cellular phenotypes (e.g. hematopoietic, neural, renal, mesenchymal). Aimed at clinical translation, the routines applied use GMP-compatible protocols and non-genome integrating reprogramming technologies. Moreover, hands-on training and student internships are offered to convey the fundamentals of stem cell research methodology. In close exchange with the complementary units within the Institute for Cell and Gene Therapy, experimental gene and cell therapeutic paradigms exploiting iPS cells are being developed.