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CAU - Microbiome Platform (CAU-Microbiome) Save as PDFShow print view

The Microbiome Platform at the Institute of Clinical Molecular Biology (IKMB) and the Institute of Experimental Medicine (IEM) combines correlative microbiome studies using high-throughput sequencing with the functional characterization of microbial components. Multi-OMIC studies conducted here, integrate microbial profiles, metabolic, genetic, phenotypic, and nutritional data, are performed using standardized high-throughput methods to identify relevant factors and mechanisms related to microbiome-host interaction. Specifically, high-throughput methods (biobanking, sample preparation, nucleic acid extraction, and sequencing-based diversity analyses) are combined with cultivation and whole-genome sequencing approaches to identify microbes and their metabolic capabilities. In addition, a strain collection of microbes associated with humans is being established. Based on previous findings and the overall objective of the studies, bioreactors (Infors HT Multifors 2) can also be used to perform physiological tests to investigate the growth kinetics, substrate utilization, and metabolic by-products of individual microorganisms under different conditions.
Address: Rosalind-Franklin-Straße 12 / Schwanenweg 20
24105  Kiel
Schleswig-Holstein
Deuschland
To website
 
  • Host Institution

Christian-Albrechts-Universität Kiel
Christian-Albrechts-Platz 4
24118  Kiel
Schleswig-Holstein
Deutschland
https://www.uni-kiel.de
Universitätsklinikum Schleswig-Holstein
Arnold-Heller-Straße 3
24105  Kiel
Schleswig-Holstein
Deutschland
https://www.uksh.de
 
  • Scientific Domain

Primary Subjects:
  • Biology
  • Medicine
Secondary Subjects:
  • Agriculture, Forestry, Horticulture and Veterinary Medicine
 
  • Category

Genomic, Transcriptomic, Proteomics and Metabolomics Facilities
 
  • Scientific Services

The Microbiome Platform offers services in the field of consulting, implementation, and evaluation of issues related to the determination of microbial diversity using high-throughput sequencing and the functional characterization of microbes within the framework of scientific cooperation. The platform's services include project planning (strategic planning, initialization of sample collection), subsequent coordination of the technical implementation of the project (sample logistics, evaluation of quality controls, troubleshooting, etc.), and the selection and implementation of suitable methodology (extraction of nucleic acids, investigation of microbial diversity via high-throughput amplicon and metagenome sequencing, cultivation and whole genome sequencing for the identification of microbes in a specific sample, establishment of a strain collection of associated microbes, determination of the metabolic capabilities of isolated microbes and complex microbial communities. Furthermore, support is offered for the bioinformatic evaluation of generated sequencing data, also with regard to the publication of the results in suitable journals.
 
  • Scientific Equipment

  • QIAcube Connect (3x)
  • SpeedMill PLUS (2x)
  • PCR Clean Bench (2x)
  • QIAxcel
  • Tecan Freedom Evo 150
  • LightCycler® 480 Instrument II
  • Anaerobic Chamber
  • Bioreactor (4x)
  • Incubators (2x)
  • Tape Station
  • Fluorescent Plate reader
  • Ultracentrifuge
 
  • Keywords

  • Microbiome
  • Microbiota
  • Amplicon-Sequencing
  • Metagenomik
  • Next-Generation-Sequencing
  • Stool samples
  • Swab samples
  • Cultivation
  • Characterisation
  • Biobank
  • OneHealth
 
  • Networks

DFG NGS competence network (NGS-CN)
https://ngs-kn.de/
Global Microbiome Conservancy (GMbC)
https://microbiomeconservancy.org/
 
  • Users per annum

Internal Users: 30
External Users in total: 30
External Users: 20
External Users in the EU: 8
External Users outside of EU: 2
 
 
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