Super-Resolution Confocal/Multiphoton Imaging for Multi-parametric Experimental Designs (SCI-MED) Save as PDFShow print view

The Super-Resolution Confocal/Multiphoton Imaging facility for Multi-parametric Experimental Designs (SCI-MED) was established at the Institute for Experimental Cardiovascular Medicine (IEKM) with the principal purpose of supporting experimental cardiovascular research in Freiburg. SCI-MED is also available for use by researchers from across the Medical Faculty, the wider University, and external collaborators. SCI-MED offers access to high resolution optical imaging for studying live-cell and -tissue samples. The facility is conceptualized especially for experiments with complex multi-parametric designs and it will cater specifically for the needs of those who wish to perform high resolution structure-function studies by combining 3D imaging with complex project-specific peripheral equipment for biophysical parameter control and observation, such as patch clamp, carbon fibre cell length-control, Langendorff perfusion, to name but a few examples. SCI-MED operates one inverted confocal microscope, one upright confocal/multiphoton microscope, and a dedicated image analysis workstation. The microscopes achieve sub-micrometre-resolution and provide the possibility to obtain deconvolution-based super-resolution or to measure fluorescence lifetimes. These systems are administered by a highly experienced facility manager.
Address: Elsässerstr. 2Q
79110  Freiburg
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  • Host Institution

Albert-Ludwigs Universität Freiburg, Universitätsklinik, Medizinische Fakultät
Breisacher Straße 153
79110  Freiburg
Universitäts-Herzzentrum Freiburg – Bad Krozingen
Hugstetter Straße 55
79106  Freiburg
  • Scientific Domain

Primary Subjects:
  • Biology
  • Medicine
Secondary Subjects:
  • Physics
  • Category

Biomedical Imaging Facilities
  • Scientific Services

The SCI-MED facility offers services for scientific enquiries in live-cell and -tissue imaging: in advance discussion of planned microscopy experiments (optimal method, sample preparation, choice of adequate fluorophores, lasers, filters etc.); basic practical introduction to the confocal or the multiphoton microscope; assistance/supervision in high-end-microscopy (e.g. FLIM, super-resolution); if required: support in optimizing experimental protocols and preparations; assistance in use of image processing software and algorithms; planning, installation and eventually performance of multi-parametric experiments (e.g. combination patch clamp / confocal); technical maintenance; scheduling and organization, invoicing; organization of workshops and training programmes; development and implementation of novel techniques, implementation of research projects; coordination with the Life Imaging Center of the University of Freiburg (facility rules, access, charges).
  • Scientific Equipment

  • Inverted confocal microscope (Leica TCS SP8X)
  • Upright confocal/multiphoton microscope (Leica TCS SP8 DIVE)
  • Image analysis workstation
  • Keywords

  • Confocal microscopy
  • Multiphoton microscopy
  • Multi-parametric experiments
  • Optogenetics
  • FLIM
  • Second-harmonic-generation imaging
  • Super-resolution
  • Networks

Microscopy and Image Analysis Platform Freiburg (MIAP-Freiburg)
  • Users per annum

Internal Users: 30
External Users in total:
External Users:
External Users in the EU:
External Users outside of EU:
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