The Super-Resolution Confocal/Multiphoton Imaging facility for Multi-parametric Experimental Designs (SCI-MED) was established at the Institute for Experimental Cardiovascular Medicine (IEKM) with the principal purpose of supporting experimental cardiovascular research in Freiburg. SCI-MED is also available for use by researchers from across the Medical Faculty, the wider University, and external collaborators. SCI-MED offers access to high resolution optical imaging for studying live-cell and -tissue samples. The facility is conceptualized especially for experiments with complex multi-parametric designs and it will cater specifically for the needs of those who wish to perform high resolution structure-function studies by combining 3D imaging with complex project-specific peripheral equipment for biophysical parameter control and observation, such as patch clamp, carbon fibre cell length-control, Langendorff perfusion, to name but a few examples. SCI-MED operates one inverted confocal microscope, one upright confocal/multiphoton microscope, and a dedicated image analysis workstation. The microscopes achieve sub-micrometre-resolution and provide the possibility to obtain deconvolution-based super-resolution or to measure fluorescence lifetimes. These systems are administered by a highly experienced facility manager.