Genomic, Transcriptomic, Proteomics and Metabolomics Facilities

The service covers compounds ranging from small molecules (Metabolomics) to large polypeptides (Proteomics). To analyze metabolites the polar fraction of a sample is measured by GC-TOF-MS, fragmented and annotated using databases. Unknown compounds are evaluated as well and can be assigned to substance classes. In addition an identification using standards can be done. The non-polar fraction is analyzed by LC-timsTOF-MS/MS. An structure elucidation of single compounds is possible as well, performing additional fragmentation experiments. Lipid analysis (Lipidomics) is also possible. Large biomolecules / proteins are routinely proteolytic digested to obtain the peptide signature. These are analyzed via nano-LC-QTOF-MS/MS. Software tools like MaxQuant or ProteinScape are used for protein identification. MSBioLMU can analyze several matrices; from single gel bands or spots up to high complex cell extracts.