Details
Mass Spectrometry of Biomolecules at LMU (MSBioLMU)


The service unit >>Mass Spectrometry of Biomolecules at LMU (MSBioLMU)<< is part of the Department Biology I of the LMU Munich.
The service can be used by internal working groups, as well as by external research establishments. A range from small molecules
/ metabolites (Metabolomics) to large polypeptides (Proteomics) are detected and quantified routinely in different matrices.
To this end, the service unit employs several mass spectrometers and chromatographs. Currently we are using a QTOF-MS/MS,
a timsTOF-MS/MS and two GC-TOF-MS. Compounds are separated by gas or liquid chromatography (GC, LC, nano-LC). But also an
analysis without any separation is possible. The service includes sample measurements, advisory service in experimental design
and data exploration. To analyze the data, several software tools and mass spectral libraries are used.
Address: Großhaderner Str. 2-4
82152 Planegg-Martinsried
Bayern
Deutschland
To website
82152 Planegg-Martinsried
Bayern
Deutschland
To website
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Host Institution
Ludwig-Maximilians-Universität München
Geschwister-Scholl-Platz 1
80539 München
Bayern
Deutschland
http://www.uni-muenchen.de
Geschwister-Scholl-Platz 1
80539 München
Bayern
Deutschland
http://www.uni-muenchen.de
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Scientific Domain
Primary Subjects:
- Biology
- Chemistry
Secondary Subjects:
- Medicine
- Agriculture, Forestry, Horticulture and Veterinary Medicine
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Category
Genomic, Transcriptomic, Proteomics and Metabolomics Facilities
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Scientific Services
The service covers compounds ranging from small molecules (Metabolomics) to large polypeptides (Proteomics).
To analyze metabolites
the polar fraction of a sample is measured by GC-TOF-MS, fragmented and annotated using databases. Unknown compounds are evaluated
as well and can be assigned to substance classes. In addition an identification using standards can be done. The non-polar
fraction is analyzed by LC-timsTOF-MS/MS. An structure elucidation of single compounds is possible as well, performing additional
fragmentation experiments. Lipid analysis (Lipidomics) is also possible.
Large biomolecules / proteins are routinely proteolytic
digested to obtain the peptide signature. These are analyzed via nano-LC-QTOF-MS/MS. Software tools like MaxQuant or ProteinScape
are used for protein identification. MSBioLMU can analyze several matrices; from single gel bands or spots up to high complex
cell extracts.
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Scientific Equipment
- nano-LC-QTOF-MS/MS
- LC-timsTOF-MS/MS
- GC-TOF-MS
- GC-TOF-MS
- HPLC
- nano-UHPLC
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Keywords
- Mass Spectrometry
- Metabolism
- Metabolomics
- Proteomics
- Plant Physiology
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Networks
SFB TR175
https://www.tr175.bio.lmu.de/
https://www.tr175.bio.lmu.de/
PhotoRedesign (Redesigning the Photosynthetic Light Reactions)
https://cordis.europa.eu/project/id/854126/de
https://cordis.europa.eu/project/id/854126/de
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Users per annum
Internal Users: 15
External Users in total: 17
External Users: 15
External Users in the EU: 2
External Users outside of EU: 0