The Core Facility Live Cell Imaging Mannheim, LIMA offers confocal and multi-photon laser scanning microscopy-related services at the Center of Biomedicine and Medical Technology Mannheim (CBTM) of the Medical Faculty Mannheim of the University Heidelberg. Amongst the equippment with high-end instruments the Core Facility LIMA offers expertise in laserscanning microscopy related issues. The service comprises in-depth training of users and support at the instruments. Moreover we support the users in optimizing their probes and the subsequent analysis of image data using various image analysis programs.