The Facility for Extracellular Vesicle Analysis and Liquid Biopsy (EV-Core) is a cross-faculty platform at the Medical Center – University of Freiburg dedicated to the analysis of extracellular vesicles (EVs) and the development of innovative biomarker strategies derived from liquid biopsy. It provides infrastructure and methodological support for scientific and clinical research projects. The aim of the facility is to support translational research projects focused on the identification and validation of EV-based biomarkers in clinical contexts such as infectiology, oncology, immuno-oncology, neurology, psychiatry, and others. In addition, it contributes to basic research on the biological roles and functions of EVs in both physiological and pathological processes. The focus lies on standardized EV isolation, single-vesicle analytics, and multi-analyte profiling of protein-, RNA-, and DNA-based components for the identification of disease-specific signatures – particularly in situations where tissue biopsies are not possible. The EV-Core combines long-standing expertise in EV research, molecular methodology, and technological innovation – positioned at the interface between experimental science and clinical application.