Genomic, Transcriptomic, Proteomics and Metabolomics Facilities

MSBioLMU offers analyses of metabolites (metabolomics) and proteins (proteomics). The polar fraction of a sample is fragmented using GC-TOF-MS (Leco, Pegasus HT) and annotated with databases. Unknown compounds are classified as "Unknown" and can be assigned to substance classes based on fragmentation patterns. Identification through standard matching is also possible. The nonpolar fraction is analyzed using LC-timsTOF-MS/MS (Bruker, timsTOF), enabling structural elucidation of individual compounds through additional fragmentation. Pigment and fatty acid analysis (lipidomics) are also available. Proteins are proteolytically digested, and peptides are analyzed using nano-LC-QTOF-MS/MS (Bruker, Impact II) or nano-LC-timsTOF-MS/MS (Bruker, timsTOF HT). Identification is performed with MaxQuant. Various sample matrices are analyzed, ranging from individual gel bands or gel spots to highly complex cell lysates or cell extracts.